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Journal: iScience
Article Title: Transient titin-dependent ventricular defects during development lead to adult atrial arrhythmia and impaired contractility
doi: 10.1016/j.isci.2024.110395
Figure Lengend Snippet: ttna Δ9/Δ9 zebrafish embryos exhibit ANP upregulation and ANP-dependent changes in I Ks channel components and atrial function (A) Quantification of mRNA levels for nppa , kcnq1 , and kcne1 in zebrafish embryos at 48 hpf. Each dot represents a biological replicate, and each replicate contains 5–10 embryos. (B) Quantification of mRNA levels for kcne1 in negative control morpholino (Control MO)-treated or morpholino targeting nppa ( nppa MO)-treated ttna Δ9/Δ9 zebrafish embryos at 48 hpf. Each data point represents a biological replicate, and each replicate contains 5–10 embryos. (C) Quantification for atrial contraction of Control MO-treated ( n = 47) or nppa MO-treated ( n = 38) ttna Δ9/Δ9 zebrafish embryos at 48 hpf. (D) Quantification for atrial contraction of Control MO-treated ( n = 19) or nppa MO-treated ( n = 17) ttna +/+ zebrafish embryos at 48 hpf. (E) Quantification of ventricular contraction of Control MO-treated ( n = 26) or nppa MO-treated ( n = 17) ttna Δ9/Δ9 zebrafish embryos at 48 hpf. Data are represented as mean ±95% confidence intervals. ns p > 0.05; ∗ p < 0.05; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001. See also Figures S9 and .
Article Snippet: anti-KCNQ1 polyclonal (rabbit) , Abcam , Cat#ab181798.
Techniques: Negative Control, Control